 |
 |
 |
 |
Is that at 10^6/mL cell concentration?
Daniel
CDC
Atlanta, GA
------------------------------------------------------------------------------
REPLY FROM: Libutti, Daniel A.
Return-Path: <owner-cyto-sendout@flowcyt.cyto.purdue.edu>
Received: from msmail by SmtpIn.em.cdc.gov id <31495BCE@SmtpIn.em.cdc.gov>;
Fri, 15 Mar 96 16:00:14 EST
Received: from flowcyt.cyto.purdue.edu by msmail (5.0/SMI-SVR4) id AA05915;
Fri, 15 Mar 1996 16:01:25 -0500
Received: by flowcyt.cyto.purdue.edu (950215.SGI.8.6.10/930416.SGI.AUTO) for
cyto-sendout id OAA01508; Fri, 15 Mar 1996 14:35:36 -0500
Received: from gemini.oscs.montana.edu by flowcyt.cyto.purdue.edu via SMTP
(950215.SGI.8.6.10/930416.SGI.AUTO) for
<cytometry@flowcyt.cyto.purdue.edu> id NAA01157; Fri, 15 Mar 1996 13:50:18
-0500
Received: by gemini.oscs.montana.edu (5.65/DEC-Ultrix/4.3) id AA23848; Fri, 15
Mar 1996 11:48:15 -0700
Date: Fri, 15 Mar 1996 11:46:33 -0700 (MST)
From: Deborah Berglund <umbbd@gemini.oscs.montana.edu>
Subject: Re: Quantifying LIVE CELLS
To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
Cc: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
In-Reply-To: <n1385379753.71265@amgen.com>
Message-Id: <Pine.3.87.9603151133.B22399-0100000@gemini.oscs.montana.edu>
Mime-Version: 1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII
content-length: 285
A good viable cell label is Fluorescein diacetate. It is taken up by
live cells, broken down inside the cells by nonspecific esterases, at
which point it becomes fluorescent. I use a stock solution of 1mg/ml and
use 10ul per ml of cells.
Deb Berglund
Montana State University
|
|
|
|