Re: Quantifying LIVE CELLS

Dave Coder (dave@nucleus.immunol.washington.edu)
Fri, 15 Mar 96 09:23:55 -0800

I am currently writing a chapter for Current Protocols in Cytometry on the
flow cytometric determination of cell viablity. I would very much appreciate
any comments on the fluorescent probe noted below as well as any comments on
other probes that you have found useful and--importantly--not useful.
Protocols as well as example illustrations are welcome. Any comments should
be sent within the next two weeks.

Dave Coder
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David M. Coder, Ph.D.
Editor, ISAC WWW Home Page
http://nucleus.immunol.washington.edu/ISAC.html

tel. 206-685-3014
fax. 206-543-3480
e-mail: dcoder@u.washington.edu

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Seattle WA 98195-7650

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Begin forwarded message:

Date: 13 Mar 1996 18:19:06 -0800
From: "Osama Nabulsi" <Osama.Nabulsi@amgen.com>
Subject: Quantifying LIVE CELLS
To: cyto-inbox
X-Mailer: Mail*Link SMTP/QM 3.0.0

REGARDING Quantifying LIVE CELLS

I am planning to run a survival bioassay were then I can quantitate LIVE
cells only using the flowcytometer.
I called Molecular Probes and they suggested "SYTOX" which stains dead cells
only (but will not cross the membrane of live cells). Have anyone used this
stain before? Advantages or Disadvantages?
or is there a live cell staining? Suggestions......

Osama Nabulsi
AMGEN INC.
(805)447-1594

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu