CD34 cell enumerations
G R BARCLAY (100066.300@compuserve.com)
15 May 95 12:44:36 EDT
Can anyone recommend protocols and reagents for enumeration of human CD34+
cells in cord blood, also in cytokine/factor stimulated cultured leucocytes from
different sources. I am using a BD FACS Sort with a Mac (CellQuest software),
and am a newcomer to this setup and FACS in general. In preliminary experiments
I have used BD RPE-anti-CD34 and BD FITC-anti-CD45 - still playing around with
settings - lots of questions, but getting fascinated. Is CD45 an optimal marker
for total leucocyte detection - should I be going for a panel of
lineage-specific markers - any recommendations and advice on this would be
appreciated.
Also - I have years of experience with PC's (DOS, Windows) but am new to Macs -
is there any way of saving files on Mac formatted discs then reading them in
PC's, and any PC software (especially downloadable shareware) for off-line
analysis of FACS data?? We may eventually have the Mac on a LAN with the PC's,
but meantime what is the best way of getting the Mac data into a PC?
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
