Re: Neutrophil Function Assay on 24 hr old EDTA blood sample.

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>In order to investigate more patients we are evaluating the procedure
>to allow neutrophil function to be tested on 24 hr samples. I was
>wondering if there are any users that have done this? The two initial
>variables as I see it are: the choice of anticoagulant and whether
>neutrophil activation can be minimized by keeping blood in a plastic
>tube before testing.

I would worry about this. The half life of neutrophils is very short
in vivo or in vitro, about 11 hours. They spontaneously enter
apoptosis, so I would question whether the cells you have left after
24 hrs are representative of those you started with.

Have fun

Mike.

............................
Caroline Gordon
Dept of Rheumatology
The University of Birmingham
Birmingham B15 2TT
United Kingdom
Tel 44 (0)21 414 6780
Fax 44 (0)21 414 6794

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