Dear all, to track our transfected cells, we have to use a red fluorescent protein. We tried pDS Red N1 (Clontech), but the signal obtain on our FAcSort is really bad (FL2 ), at least as compared to what we get when we use pEGFP N1 (FL1). Is anyone aware of a "better" red FP, that is efficiently excitable with the argon laser, and displays a good and bright emission? Philippe ____________________________________________ Philippe Pognonec, Ph.D. Transcriptional Regulation and Differentiation Centre de Biochimie Parc Valrose Universite de Nice 06108 Nice cedex 2 France Tel/fax: (33) 492 07 64 13 http://www.unice.fr/biochimie/u470/prog5us.htm ____________________________________________
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