Hi there. We are using FDA as a marker of living/dead cells in thymocytes preparations on a FACSort, in FL1. We do not observe any uncontrolable leakage of the flurescence in FL2, and are pretty happy with it. Fine. No we try the same stuff on 293 cells (after dissociation following light trypsination), and we get teh same signal in FL1, 2 and 3!!!! Impossible to use any other marker at the same time. Does anybody have any idea what's wrong? Philippe ____________________________________________ Philippe Pognonec, Ph.D. Transcriptional Regulation and Differentiation Centre de Biochimie Parc Valrose Universite de Nice 06108 Nice cedex 2 France Tel/fax: (33) 492 07 64 13 http://www.unice.fr/biochimie/u470/prog5us.htm ____________________________________________
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