While this subject has come up: it seems like both 7AAD and propidium iodide can be (and are) used for detecting dead or apoptotic cells - are they interchangeable ? If people have distinct preferences, what is the difference ? thanks, Rachel p.s Happy Thanksgiving (belated) ======================================================= Rachel M. Gerstein, Ph.D. Department of Molecular Genetics and Microbiology Graduate Program in Immunology/Virology University of Massachusetts Medical School 55 Lake Avenue North Worcester, MA 01655-0002 (508) 856-1044 (508) 856-5920 (FAX) > ---------- > From: Liza McGuire > Sent: Tuesday, November 20, 2001 2:17 PM > To: Cytometry Mailing List > Subject: 7AAD PROTOCOLS > > Hi Flowers, > > Can anyone send me a good comprehensive 7AAD protocol for staining, > acquisition and analysis (gating strategies) of lymphocyte subsets. > I appreciate any help I can get. > Thank you so much, > > Liza McGuire > Thomas Jefferson University Hospital > 130 S 9th Street, Rm. 400 Edison > Philadelphia, PA 19107-5233 > (215)955-9646 >
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