Dear Rachel, 7AAD is may be better in some application because of the lower crosstalking to the FL2 channel. Accordingly you can use it together with PE stains. PI is very hard to compensate from the FL2. But if you use only FITC as second stain (and nothing else) you can use both 7AAD and PI for dead cell labeling. Pal Jakso University of Pecs Faculty of Medicine Dept. of Pathology 7643, Hungary 12. Szigeti str. While this subject has come up: it seems like both 7AAD and propidium iodide can be (and are) used for detecting dead or apoptotic cells - are they interchangeable ? If people have distinct preferences, what is the difference ? thanks, Rachel
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