Frank My advise would be to try One Lambda in Ca. 800-822-8824. They have the biggest selection of antibodies for flow in the US. Two things: 100 micoliters of blood is pushing the limits. The small quantity would be about 100uL per assay considering the isotype control. HLA-B7 is a highly cross reactive antigen so it will be difficult to detect this antigen alone by flow. Russ >From: Frank Mattes <list_server2001@yahoo.com> >To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> >Subject: help HLA-I typing >Date: Fri, 23 Nov 2001 09:21:38 -0800 (PST) > > >Dear FACS expert, > >to begin, I'm just a beginner in the FACS world, so I >hope I'm not upsetting anyone with my question. > >For my research project, I need to know the HLA-I type > >of the patients under study. I thought the quickest >way would be to HLA type on a aliquot of the bood >sample I received. >I don't need the full HLA-type, I need only to know if >the patient is A2, B7, B8, B35. >I'm wondering if someone know a method to do that on a >small blood volume ( 100 micol)without isolating >PBMCs. > >My 2nd question is, if the Ab are comercially >available. > >I appreciate your help > >Frank Mattes >Department of Virology >Royal Free Hospital, London > >__________________________________________________ >Do You Yahoo!? >Yahoo! GeoCities - quick and easy web site hosting, just $8.95/month. >http://geocities.yahoo.com/ps/info1 _________________________________________________________________ Get your FREE download of MSN Explorer at http://explorer.msn.com/intl.asp
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