We use a very rapid and efficient method. We dilute MNC (after ficoll) in PBS+1 mM EDTA + 0,5% HSA + 5% ACD in a 50 mL tube. Then we performe a 15 seconds centifugations at 1680 g (300 RPM with our centrifuge). We repeat the procedure once or twice. It as the same results than 150 g for 10 minutes and is much less expensive than RosetteSep. Regards > > From: Dr. Christiane Hollmann [mailto:ch@adnagen.com] > Sent: Wednesday, November 21, 2001 1:51 AM > To: cyto-inbox > Subject: depletion of platelets > > Dear Flowers, > I have a problem to get rid of remaining platelets after MNC preparation via > Ficoll gradient. Up to now we centrifuge the MNC through FCS or BSA. Are > their any protocols to increase the efficiency of platelet depletion? Please > let me know! > Regards > Christiane > > ------------------------------------------------------------------------ > > Part 1.2 Type: application/ms-tnef > Encoding: base64
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