Dear Philippe, DsRed is a difficult beast to work with. Main problems are slow maturation and aggregation. We are submitting a paper on a tight link between aggregation (minimum is a dimer) and generation of a functional chromophore. I will be glad to share the results with you once we have feedback from the journal. ciao Saverio Saverio Alberti Head, Lab. of Experimental Oncology Department of Cell Biology and Oncology Consorzio Mario Negri Sud 66030 Santa Maria Imbaro (Chieti), Italy Phone: (39-0872) 570.293 FAX: (39-0872) 570.412 E-mail: alberti@cmns.mnegri.it On Mon, 26 Nov 2001, Philippe Pognonec wrote: > > Dear all, > to track our transfected cells, we have to use a red fluorescent protein. We tried > pDS Red N1 (Clontech), but the signal obtain on our FAcSort is really bad (FL2 ), at > least as compared to what we get when we use pEGFP N1 (FL1). > Is anyone aware of a "better" red FP, that is efficiently excitable with the argon > laser, and displays a good and bright emission? > Philippe > ____________________________________________ > > Philippe Pognonec, Ph.D. > Transcriptional Regulation and Differentiation > Centre de Biochimie > Parc Valrose > Universite de Nice > 06108 Nice cedex 2 > France > Tel/fax: (33) 492 07 64 13 > http://www.unice.fr/biochimie/u470/prog5us.htm > ____________________________________________ > > > > >
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