I am looking for a good positive control for IL-10 production by human peripheral blood lymphocytes, measured by FACS analysis of cell-specific cytokine production. We stimulate whole blood for 6 -18 hours; SEB and PMA/I induce good IL-2 and IFN-g, but not IL-10. I know we can use LPS to induce IL-10 production in monocytes, but I would prefer a positive control that is lymphocyte-specific. Any ideas?? Thanks! Willem Hanekom The Rockefeller University, New York.
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