Dear Willem We're having similar problems. If you do get some helpful information, I would be most grateful if you could pass it on to me. I have heard that the superantigen Staph enterotoxin B (SEB) is a good alternative positive control. Co-stimulation with anti-CD28 antibody increasesthe number of responding T cells. Hope this is of some help LINDA On Thu, 15 Nov 2001 12:19:40 -0500 Willem Hanekom <Willem.Hanekom@rockefeller.edu> wrote: > > I am looking for a good positive control for IL-10 production by human > peripheral blood lymphocytes, measured by FACS analysis of cell-specific > cytokine production. We stimulate whole blood for 6 -18 hours; SEB and PMA/I > induce good IL-2 and IFN-g, but not IL-10. I know we can use LPS to induce > IL-10 production in monocytes, but I would prefer a positive control that is > lymphocyte-specific. Any ideas?? > Thanks! > Willem Hanekom > The Rockefeller University, New York. > ---------------------- Linda Duncan Dept Ophthalmology University of Aberdeen Medical School Foresterhill Aberdeen AB25 2ZD l.duncan@abdn.ac.uk
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