Intracellular Cytokine Detection Workshop Announcement

From: Burt_Houtz@BDIS.Com
Date: Tue Nov 27 2001 - 13:15:47 EST


BD Biosciences announces the Intracellular Cytokine Detection Workshop
Course content
In Vitro activation of whole blood
Cell permeabilization and labeling techniques
Flow cytometric analysis of cytokine-producing lymphocyte subsets
Troubleshooting data and sample preparation

Students prepare samples using the BD FASTImmune CD4 Intracellular Cytokine
Detection Kit,
optimize the flow cytometer for data acquisition, and analyze acquired data.
Instrument QC
is performed using FACSComp software. Sample acquisition and analysis are
performed using
CellQuest software. Emphasis is placed on analytical interpretation of data and
troubleshooting.

Who should attend:  Users proficient in FACSCalibur/FACScan/FACSort operation
and who want
to gain experience in preparing and analyzing specimens used in studying immune
function.

Duration: 1 day

Schedule: Nov 30,  San Jose, CA
          Feb 28,  Mansfield, MA
          May 16, San Jose, CA

Prerequisites: A basic understanding of immunology and completion of an operator
course, or equivalent experience that includes data acquisition and analysis
using CellQuest software.

Tuition: $495

Contact:  Wayland Quon - (800) 451-4557 x 2010


Burt Houtz
Technical Advisor
Flow Cytometry Applications
BD Biosciences
San Jose, CA
bhoutz@bdis.com
408-954-2040



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