Dears We are working with purified PBMC after ficoll gradient and four color analyse. After gradient, some red cells are purified with the mononuclear cells and will be stained by CFSE and thus interfering with the analyse. We are now lysing red cells after surface stainig to avoid these interference. We send you typical analyse obtained in our lab before and after lising. Have a good day Thank's -- Dr Daniel SCOTT-ALGARA Service d'Immuno-hématologie et d'Immunopathologie Institut Pasteur 75724 Paris Cedex 15 France Phone 33 (0)1 45 68 82 13 Fax 33 (0) 1 45 68 89 51 E-mail: scott@pasteur.fr
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