Jeff, There is a more definitive macrophage antibody for mature mouse macs, it's name is F4/80 and is available from Caltag Laboratories. It comes in many flavors of color. Best regards from a person who did all the Stowers sorting at Caltech, Rochelle ( Shelley) Diamond Caltech Flow Cytometry Cell Sorting Facility On Mon, 29 Oct 2001, Haug, Jeff wrote: > > Dear Follow Cytometrists, > > I would like to confidently enumerating monocytes and granulocytes > in mouse marrow and peripheral blood. I am currently using MAC-1 and GR-1, > however the combination does not clearly separate the myeloid cells from > each other. Does anyone have suggestions about other antibodies, markers, or > methods that can be used for this purpose? > > Best Regards, jsh > > Jeffrey S. Haug > Stowers Institute for Medical Research > 1000 E. 50th Street > Kansas City, Missouri 64110 > 816-926-4142 > FAX 816-926-2022 > >
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