Miltenyi beads are nanparticules which do not change SSC and FSC. You wil found CD34 in tne" lymphocyte" gate. We performed CD34 purification from normal blood for 7 years with the MACS system and obtained usually 80% to 90% purity of the CD34 population (with two comumns). "Kong, Denice" wrote: > Dear flow users: > > I am currently evaluating using Miltenyi microbeads to isolate CD34 from > peripheral blood and checking the purity using flow. Has anyone done this > type of study before. If so, where would the CD34+ cells be on the SSC and > FSC plot with or without the microbeads? What purity was obtained? > > Denice Kong > UCSF-ITL
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