Hi all, How can one be sure that the sub G1 events are apoptotic events, and not just DNA fragment clusters of varying sizes? Wouldn't marker statistics of the sub-G1 'pile' also be inaccurate, as the marked events are not actually cells, but fragments of DNA? Also, regarding the presence of a sub-G1 'apoptotic' peak: what would cause apoptotic cells to all display slightly lower, yet Gaussian, DNA content? Doesn't a sub-G1 peak mean that these cells all lost the same, small amount of DNA? My apologies if this has been covered in the past. Cheers, Nate Regimbal PPD Discovery Menlo Park, CA Tim Kute wrote: > In most cases, I like to have my G1 peak start in the 1/3 rd of the x axis > range. If you are looking for apoptotic cells, you might want it higher > around 2/3 rd of range. If you really want to see every event, you might > even want to put the scale on log and have the G1 peak at the 80% area of > the x axis. > > Tim Kute > > Maciej Simm wrote: > > > I'm posting this on behalf of a collegue. She's doing an assay with > > just PI looking at the peaks before/after incubation of her cells in > > pro-apoptotic drugs. If anyone looks at PI on a Calibur, could you > > fill me in on the instrument settings that work the best? I would > > appreciate any other tips. > > > > Maciej > > > > >find out voltage settings for the PI assay. in particular ask about > > >a printout of an acquisition template they use. also - > > > > >fsc lin or log? what voltage? > > >ssc same as above > > >fsc s.a.a. > > >fl2 s.a.a. > > >fl3 s.a.a. > > > > >what about compensation? > > > > __________________________________________________ > > Do You Yahoo!? > > Make international calls for as low as $.04/minute with Yahoo! Messenger > > http://phonecard.yahoo.com/ ______________________________________________________________________ This email transmission and any documents, files or previous email messages attached to it may contain information that is confidential or legally privileged. If you are not the intended recipient or a person responsible for delivering this transmission to the intended recipient, you are hereby notified that you must not read this transmission and that any disclosure, copying, printing, distribution or use of this transmission is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by telephone or return email and delete the original transmission and its attachments without reading or saving in any manner. ______________________________________________________________________
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