Alice I have several investigators sorting single cells on our MoFlo for RT-PCR. One of them started with the method published in 5th Ed. Weir Handbook of Exp. Immunology vol1 p13. For best recovery you will want to make sure your sorted cells travel straight rather than being in an angled deflected stream. Larry At 03:09 PM 7/31/2001, you wrote: >Hello Flowers, >Not sure why I am having trouble finding this on my own, but I will >swallow my pride >and ask if someone could point me to a good methodological article or >chapter on >combining single cell flow sorting with pcr amplification? > >Thanks. > >Alice > >Alice L. Givan >Englert Cell Analysis Laboratory >of the Norris Cotton Cancer Center >Dartmouth Medical School >Lebanon, New Hampshire NH 03756 >tel 603-650-7661 >fax 603-650-6130 >givan@dartmouth.edu Larry W. Arnold, Ph.D. Res. Assoc. Prof. Director, Flow Cytometry Facility Department of Microbiology and Immunology Lineberger Comprehensive Cancer Center CB# 7290 University of North Carolina at Chapel Hill Chapel Hill, NC 27599 Phone: 919-966-1530 FAX: 919-962-8103
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