In most cases, I like to have my G1 peak start in the 1/3 rd of the x axis range. If you are looking for apoptotic cells, you might want it higher around 2/3 rd of range. If you really want to see every event, you might even want to put the scale on log and have the G1 peak at the 80% area of the x axis. Tim Kute Maciej Simm wrote: > I'm posting this on behalf of a collegue. She's doing an assay with > just PI looking at the peaks before/after incubation of her cells in > pro-apoptotic drugs. If anyone looks at PI on a Calibur, could you > fill me in on the instrument settings that work the best? I would > appreciate any other tips. > > Maciej > > >find out voltage settings for the PI assay. in particular ask about > >a printout of an acquisition template they use. also - > > >fsc lin or log? what voltage? > >ssc same as above > >fsc s.a.a. > >fl2 s.a.a. > >fl3 s.a.a. > > >what about compensation? > > __________________________________________________ > Do You Yahoo!? > Make international calls for as low as $.04/minute with Yahoo! Messenger > http://phonecard.yahoo.com/
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