mRNA in bacteria

From: Elena Soriano (E.Soriano@iam.boku.ac.at)
Date: Mon May 07 2001 - 07:56:20 EST

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I´m trying to detect a specific mRNA fragment in bacteria by in situ
hybridization. Does somebody have any experience in this?

I´m afraid to find some troubles between the best way to permeabilizate the
bacteria to the labelled probe complex and the preservation of the RNA...
What kind of labelled probes or oligos could I use (in matter of size and
labelling), and where could I purchase them?
I´m quite lost at the moment...

Thank you in advance for your interess.


Elena.




-----------------------------------------
Elena Soriano
c/o Institut für Angewandte Mikrobiologie
    Univ.f.Bodenkultur Wien

Tel: +43 1 36006 6241
Fax: +43 1 36 97 615
http://www.boku.ac.at/iam

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