One of my colleagues replied as follows:
People are starting to use in situ hybridization to detect/characterize H.
pylori in biopsy specimens, though they typically detect rRNA, not mRNA. I
don't know if this would make a difference. For most of the papers I have
read, they either make their own probes or have them made.
A recent paper is Russmann et al, J. Clin. Microbiol 39:304-308 (2001). (It
was published in January, so should be available on the ASM website without
a subscription when the July issue comes out).
That paper refers to a J. Bacteriology paper with pretty detailed methods
(volume 172, pages 762-770, 1990--this is why I have kept my old J.
Bacteriology journals!). I don't know how current this paper is though.
This is not an area I know a lot about, so this is just what I could find
easily.
-----Original Message-----
From: Elena Soriano [mailto:E.Soriano@iam.boku.ac.at]
Sent: Monday, May 07, 2001 8:56 AM
To: cyto-inbox
Subject: mRNA in bacteria
I´m trying to detect a specific mRNA fragment in bacteria by in situ
hybridization. Does somebody have any experience in this?
I´m afraid to find some troubles between the best way to permeabilizate the
bacteria to the labelled probe complex and the preservation of the RNA...
What kind of labelled probes or oligos could I use (in matter of size and
labelling), and where could I purchase them?
I´m quite lost at the moment...
Thank you in advance for your interess.
Elena.
-----------------------------------------
Elena Soriano
c/o Institut für Angewandte Mikrobiologie
Univ.f.Bodenkultur Wien
Tel: +43 1 36006 6241
Fax: +43 1 36 97 615
http://www.boku.ac.at/iam
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