All, I too think that time has come for a Center for Microbial Cytometry. Howard would make a great director. I would be interested in serving in some leadership capacity as well. I have spent the last 5 yrs looking at many of these same issues and a group like this would be very helpful. Regards, Joseph Martinez Research Biologist Director Flow Cytometry Laboratory Respiratory Diseases Branch/Immunology Section Centers for Disease Control and Prevention Atlanta,GA -----Original Message----- From: Warnock, David Sent: Wednesday, May 09, 2001 10:13 AM To: cyto-inbox Subject: FW: mRNA in bacteria -----Original Message----- From: Nicholson, Janet Sent: Wednesday, May 09, 2001 8:49 AM To: cyto-inbox Schable, Charles; Tenover, Fred; Vafai, Abbas; Vorndam, Vance; Warnock, David Subject: FW: mRNA in bacteria Could you distribute this to your laboratorians? Howard Shapiro is considered one of the most knowledgeable people when it comes to flow cytometry, and he has a keen interest in detecting organisms. Let me know if anyone expresses an interest in his idea. -----Original Message----- From: Howard Shapiro [mailto:hms@shapirolab.com] Sent: Tuesday, May 08, 2001 11:04 PM To: cyto-inbox Subject: RE: mRNA in bacteria Jan- The original posting on this subject on the Mailing List and your reply to it provided two more reminders that the state of cytometry for bacteria, fungi, and viruses is fairly primitive and its rate of progress is way too slow. Most instruments are not optimized for dealing with microbes, many dyes behave substantially differently in microorganisms and eukaryotic cells, and there are not a lot of specific (antibody and nucleic acid probe) reagents available. During the past couple of months, I have come to the conclusion that there needs to be a Center for Microbial Cytometry to address these issues, initially by facilitating communication and collaboration among the relatively small number of researchers in this area, and later by either galvanizing the industry into meeting the need for instruments and reagents or arranging for production and distribution in some kind of nonprofit institutional setting. Since I'm spending an increasing fraction of my time measuring bacteria and trying to measure viruses, I've nominated myself to be the Director. I'd welcome input on this subject from you and from interested parties at CDC. By the way, did you ever get around to reading "Microbe Hunters"? I'd be happy to send you one of my copies. -Howard
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