>>> Scott Tighe <stighe@zoo.uvm.edu> - 3/15/2001 3:41 PM >>> Greeting all: I have a situation in which I am detecting a cytokine that its expression is low. My question is, we are using PE as our secondary stain and I am wondering if I use a brighter stain if my negative and positive peaks will separate more? Is this a reasonable assumption and if so, what stain would be best? Scott Tighe Vermont Cancer Center
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