I found minimal toxicity using PKH26 on BMMC/CD34+ cells when cultured directly after staining. HOWEVER photoxicity at high doses of PKH26 may be a problem after excitation light exposure for sorting, cell viability may drop (see Duk Jae Oh et al, Cytometry 36:312-318, 1999). ----------------------------------- sian rizzo @sghms.ac.uk Haematology, St. Georges Hospital Medical School Opinions expressed those of the author and not the institution
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