Check out the enzymic amplification technique described by David Kaplan et al. Cytometry 40 (1) May 2000 page 81. We have had great success with it detecting Fas Ligand, increase your signal a lot without an increase in backgroound. Simon Monard FACS Lab Manager Trudeau Institute Saranac Lake NY12983 Ph 518 891 3080 X352 >>> Scott Tighe <stighe@zoo.uvm.edu> - 3/15/2001 3:41 PM >>> Greeting all: I have a situation in which I am detecting a cytokine that its expression is low. My question is, we are using PE as our secondary stain and I am wondering if I use a brighter stain if my negative and positive peaks will separate more? Is this a reasonable assumption and if so, what stain would be best? Scott Tighe Vermont Cancer Center
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