Secondary staining

From: Scott Tighe (stighe@zoo.uvm.edu)
Date: Thu Mar 15 2001 - 15:41:18 EST 

Greeting all:

I have a situation in which I am detecting a cytokine that its
expression is low. My question is, we are using PE as our secondary
stain and I am wondering if I use a brighter stain if my negative and
positive peaks will separate more? Is this a reasonable assumption and
if so, what stain would be best?

Scott Tighe
Vermont Cancer Center

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