That's interesting. I recall a discussion at MUG with somebody that indicated that if they didn't use enough media in their plates the cells got stuck to the bottom. Perhaps, they got stuck on the bottom, but didn't die/splat. I assumed that they went splat. learn something new every day. time to go home :) chris PS. achieved quota of learning today's one new thing very late in the day.. On Tue, 23 Jan 2001 13:23:35 -0500, Larry Arnold wrote: > >Works fine. Cells will be a bit dilute. I have sorted with my MoFlo >onto a slide put on a cover slip and gone straight to my LSC. Cells are >fine. Usually put about 1000 cells down for a 22mm2 cover slip suing the >150um tip so could do more with a 70 or 100um tip. > >Larry > >At 03:09 PM 1/22/2001 -0500, you wrote: > >>One of my colleagues here was asking me if it was possible to high speed >>sort onto a glass slide. I've sorted into microtitre plates but never tried >>slides....anyone tried this? >> >>if so how bad was the charge build up from the stream? >>were the cells still viable or do they go SPLAT? >> >>Cj Jett >>Scientist: Biomedical Development >>Compucyte Corp. >>(617)-577-3811 >>12 Emily St. >>Cambridge Ma 02139-4507 > >Larry W. Arnold, Ph.D. >Res. Assoc. Prof. >Director, Flow Cytometry Facility >Department of Microbiology and Immunology >Lineberger Comprehensive Cancer Center >CB# 7290 >University of North Carolina at Chapel Hill >Chapel Hill, NC 27599 >Phone: 919-966-1530 >FAX: 919-962-8103 >
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