I routinely sort stuff onto a slide to check that my sorts are working (a picture is worth a thousand words). Just put a drop of PBS on the slide and away you go. As far as high speed, I can't see why you would need high speed for the small number of drops you can fit on a slide (few thousand) let alone put a cover slip on without it swimming away. Viability of the cells should be fine as long as there is some PBS on the slide to begin with, and that you don't take too long to look at them. I have done this most recently with hela cells without much difficulty. At 03:09 PM 1/22/01 -0500, you wrote: >One of my colleagues here was asking me if it was possible to high speed >sort onto a glass slide. I've sorted into microtitre plates but never tried >slides....anyone tried this? > >if so how bad was the charge build up from the stream? >were the cells still viable or do they go SPLAT? > >Cj Jett >Scientist: Biomedical Development >Compucyte Corp. >(617)-577-3811 >12 Emily St. >Cambridge Ma 02139-4507 Derek Schulze Cancer Research Labs Queen's University 353 Botterell Hall Kingston, ON, K7L 3N6 (613) 533-6635 http://meds.queensu.ca/medicine/crl/
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:01:03 EST