Howard I typical run this tip at 10-12 PSI. DDF is 14-16K. I usually limit the sort speed to 33% of DDF so would try not to exceed 5K/s (unless of course I'm wanting to go very fast in an enrichment mode - have done 10K/s on the 150 tip). The calculated cell velocity would be around 10-11m/s. Larry At 08:53 PM 1/25/2001 -0500, you wrote: >Larry- > >>Works fine. Cells will be a bit dilute. I have sorted with my MoFlo >>onto a slide put on a cover slip and gone straight to my LSC. Cells are >>fine. Usually put about 1000 cells down for a 22mm2 cover slip suing the >>150um tip so could do more with a 70 or 100um tip. > >I guess I never thought about using a 150 um tip on a high speed >sorter. How many cells a second can you analyze with that big a stream, >and what is the flow velocity? > >Thanks, > >-Howard > Larry W. Arnold, Ph.D. Res. Assoc. Prof. Director, Flow Cytometry Facility Department of Microbiology and Immunology Lineberger Comprehensive Cancer Center CB# 7290 University of North Carolina at Chapel Hill Chapel Hill, NC 27599 Phone: 919-966-1530 FAX: 919-962-8103
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