Re: High speed sorting onto a glass slide

From: Larry Arnold (lwarma@med.unc.edu)
Date: Tue Jan 23 2001 - 13:23:35 EST


Works fine.  Cells will be a bit dilute.    I have sorted with my MoFlo
onto a slide put on a cover slip and gone straight to my LSC.  Cells are
fine.  Usually put about 1000 cells down for a 22mm2 cover slip suing the
150um tip so could do more with a 70 or 100um tip.

Larry

At 03:09 PM 1/22/2001 -0500, you wrote:

>One of my colleagues here was asking me if it was possible to high speed
>sort onto a glass slide.  I've sorted into microtitre plates but never tried
>slides....anyone tried this?
>
>if so how bad was the charge build up from the stream?
>were the cells still viable or do they go SPLAT?
>
>Cj Jett
>Scientist: Biomedical Development
>Compucyte Corp.
>(617)-577-3811
>12 Emily St.
>Cambridge Ma 02139-4507

Larry W. Arnold, Ph.D.
Res. Assoc. Prof.
Director, Flow Cytometry Facility
Department of Microbiology and Immunology
Lineberger Comprehensive Cancer Center
CB# 7290
University of North Carolina at Chapel Hill
Chapel Hill, NC 27599
Phone: 919-966-1530
FAX: 919-962-8103



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