Hi Henry, the amount you wrote expands to 10 nanomolar per ml: the nanomole per litre per millilitre is a pretty unusual unit - I imagine that they meant 10 nanomoles per ml (which is equivalent to 10 micromoles per litre or ten micromolar - which seems fair), but used the "M" by accident when they meant "mole". Ray > From: "Henry H. Wortis" <henry.wortis@tufts.edu> > Organization: Tufts University School of Medicine > Date: Mon, 22 Jan 2001 19:43:52 -0500 > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > Subject: CFSE > > > Would someone with experience using CFSE, particularly with murine B cells, > confirm > the correct dose? The one published > study with B cells that I found states that a dose of 10 nM/ml was used. This > seems > to be about 1000 fold less than the > usual dose. > > > Henry H. Wortis, M.D., Professor > Department of Pathology, > Director, Graduate Program in Immunology > Tufts University School of Medicine > 136 Harrison Ave. > Boston MA 02111 > Phone 617-636-6718 > FAX 617-636-2990 > > >
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