I have enriched for murine DCs by both sequential enrichment and magnetic seperation. However I do not have any expereience with sorting. We did find that magenetic seperation with CD11c produced a substatial proportion of macrophage cells. Let me know if I can be any help. Janet On Thu, 14 Dec 2000, Samuel Lundin wrote: > > Hello all, > > Has anyone of you any experince of sorting mouse spleen dendritic cells > (CD11c-expressing)? > I am going to attempt to sort such cells (for T-cell stimulation > experiments), and would like to get some information about possible > pitfalls, etc. > > I am especially interested in what pressures and nozzle sizes you have been > using. > > Regards, > Samuel Lundin > ============================================ > Samuel Lundin, PhD > Dept. of Medical Microbiology and Immunology, > Göteborg University > Box 435, 405 30 Göteborg, Sweden > Phone: +46-31- 3424685 / 3424716 > Fax: +46-31-826976 >
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