Sorting of mouse dendritic cells

From: Samuel Lundin (samuel.lundin@microbio.gu.se)
Date: Thu Dec 14 2000 - 08:35:58 EST


Hello all,

Has anyone of you any experince of sorting mouse spleen dendritic cells
(CD11c-expressing)?
I am going to attempt to sort such cells (for T-cell stimulation
experiments), and would like to get some information about possible
pitfalls, etc.

I am especially interested in what pressures and nozzle sizes you have been
using.

Regards,
Samuel Lundin
============================================
Samuel Lundin, PhD
Dept. of Medical Microbiology and Immunology,
Göteborg University
Box 435, 405 30 Göteborg, Sweden
Phone: +46-31- 3424685 / 3424716
Fax: +46-31-826976



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