Samuel, We have sorted mouse DCs regularly on a MoFlo at 55psi with a 70 micron nozzle and on a FACS II or a FACStar+ at 13-15psi, also with 70 micron nozzles. We haven't discovered any pitfalls specific for DCs. BTW, functional assays don't show any ill effects of the higher pressure sorts. Frank. Frank Battye \ / < The Cytometry Laboratory Dora Kaminaris \__/ <<<<< The Walter & Eliza Hall Institute Viki Lapatis ------!!<<<<<<<< Victoria 3050, AUSTRALIA Jennie Chan /!!\ <<<<< ph: 61_3_9345 2540 fax: 61_3_9347 0852 Catherine Tarlinton o !! \ < in:: "facs_copy@wehi.edu.au" Geza Paukovics / !! \
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