Hi Warren. It's the APC beads. I don't know what the deal is but they crap out in a hurry. I think the normal target value for the APC beads is about 212. Go into the FACSComp setup and change the target value to about 175. You were receiving that error message because you aren't getting enough signal out of the APC beads for them to be recognized. You could get some new APC beads, however, they will probably lose intensity quickly so you might as well know how to do this anyway. If these suggestions don't do the trick it could actually be the laser. I had one fail after about 3 years. David McFarland SmithKline Beecham Pharmaceuticals ---------------------- Forwarded by David C McFarland/DEV/PHRD/SB_PLC on 06/27/2000 16:58 --------------------------- wds8@email.med.yale.edu on 26-Jun-2000 17:07 To: cyto-inbox cc: (bcc: David C McFarland/DEV/PHRD/SB_PLC) Subject: problems with time delay calibration on FACS Calibur Dear Flowers (in particular FACS Calibur users), We have a new FACS Calibur and have had persistent problems with reproducibly being able to set time delay calibration. In particular, we get the error message, "signal out of range...unable to set time delay." BD has replaced an EPROM and a board, but the problem intermittently persists. In speaking to users of 3 other Caliburs I discovered that they too had seen this problem. I'd appreciate any comments from other two laser users on the Calibur and will summarize the responses to the list. Many thanks. Warren Warren D. Shlomchik, M.D. Assistant Professor of Medicine Yale University School of Medicine Section of Medical Oncology 333 Cedar Street WWW217 New Haven, CT 06520 phone:(203)737-2478 FAX: (520)395-8571
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:24 EST