Hi Warren, In addition to the comments below, I'd just like to mention that we also see this problem occasionally. We have to use a threhlod FSC value of 200 to be able to do any time delay calibration at all with the APC beads. In addition, whenever this problem occurs now, it is usually because there is a problem with the FACS buffers reservoirs/tubings: either the caps is not on or there is an air bubble in the tubings etc. We seem to get around this well by ensuring the cap is on nice and tight and doing a couple of primings. Good luck. Francis Karanu >Hi Warren. It's the APC beads. I don't know what the deal is but they >crap out >in a hurry. I think the normal target value for the APC beads is about >212. Go >into the FACSComp setup and change the target value to about 175. You were >receiving that error message because you aren't getting enough signal out >of the >APC beads for them to be recognized. You could get some new APC beads, >however, >they will probably lose intensity quickly so you might as well know how to do >this anyway. If these suggestions don't do the trick it could actually be the >laser. I had one fail after about 3 years. > >David McFarland >SmithKline Beecham Pharmaceuticals > > >---------------------- Forwarded by David C McFarland/DEV/PHRD/SB_PLC on >06/27/2000 16:58 --------------------------- > > >wds8@email.med.yale.edu on 26-Jun-2000 17:07 > > > >To: cyto-inbox >cc: (bcc: David C McFarland/DEV/PHRD/SB_PLC) >Subject: problems with time delay calibration on FACS Calibur > > > > > >Dear Flowers (in particular FACS Calibur users), > >We have a new FACS Calibur and have had persistent problems with >reproducibly being able to set time delay calibration. In >particular, we get the error message, "signal out of range...unable >to set time delay." BD has replaced an EPROM and a board, but the >problem intermittently persists. In speaking to users of 3 other >Caliburs I discovered that they too had seen this problem. I'd >appreciate any comments from other two laser users on the Calibur and >will summarize the responses to the list. Many thanks. > > >Warren > > >Warren D. Shlomchik, M.D. >Assistant Professor of Medicine >Yale University School of Medicine >Section of Medical Oncology >333 Cedar Street WWW217 >New Haven, CT 06520 > >phone:(203)737-2478 >FAX: (520)395-8571
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