--- You wrote: Another reference for the PKH26 method is A.L. Givan et al, J. Immunol. Methods, 1999, 230:99-112. --- end of quote --- Beware the misprint in the formula for precursor frequency in this article. There should be a line between the two components in brackets (that is, the first bracketed component is divided by the second bracketed component). Actually, the newest version of ModFit includes this calculation of precursor frequency --- so you don't need to use the formula. Alice Givan
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