proliferative responses

From: Jose Benito (jbenito1@hotmail.com)
Date: Tue Sep 19 2000 - 14:10:24 EST


Hi everyone, I am trying to set up a colorimetric assay to measure
lymphoproliferative responses to mitogens and recall antigens. I have tried
the Alamar Blue without any sucess. I will probably try also the celltiter
assay from promega. Does anyone have experience with this kind of assays?
Can they really substitute the 3Hthymidine assay?
Thanks
Jose Miguel
Hospital Carlos III
Madrid
Spain
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