Another reference for the PKH26 method is A.L. Givan et al, J. Immunol. Methods, 1999, 230:99-112. I was unable to find the J.I. article referred to; it can't be volume 214 in 1998-can you provide the authors? Thanks, Mandy >Jose Benito, >Yes there is such a method. Its based on PKH26 dye that is incorporated >stably into the membrane of cells. As cells proliferate in response to >stimulation, you get a reduction in the individual cell fluorescence. The >main advantage of this assay is that it can be combined with other >phenotypic markers to determine proliferation of individual subpopulation.. >See: Journal of Immunology: 1998; 214:175-186. > >John Waitumbi Mandy
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