Jose Benito, Yes there is such a method. Its based on PKH26 dye that is incorporated stably into the membrane of cells. As cells proliferate in response to stimulation, you get a reduction in the individual cell fluorescence. The main advantage of this assay is that it can be combined with other phenotypic markers to determine proliferation of individual subpopulation.. See: Journal of Immunology: 1998; 214:175-186. John Waitumbi ------------------------------------------------------------------------ Dr. John N. Waitumbi Walter Reed Project P.O. Box 30137 Nairobi Kenya Phone +254 35 22942 Email:waitumbi@net2000ke.com Fax +254 35 22903 Electronic Fax Service: 1-603- 947-4913 ----- Original Message ----- From: Jose Benito <jbenito1@hotmail.com> To: cyto-inbox Sent: Tuesday, June 20, 2000 9:51 AM Subject: proliferative responses > > Hi eveyone. Sorry, this is not a flow question but does anyone know of a > fluorimetric-based technique to measure lymphoproliferative responses in > response to mitogens and antigens?. Molecular Probes has a kit called > "Cyquant" that measures DNA content using a DNA-binding green dye. Does > anyone have some experience using this kit? > Thanks > Jose Miguel Benito > Hospital Carlos III > Madrid > Spain > ________________________________________________________________________ > Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com >
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