>From what I can gather it sounds like you are using a 60Hz AC light source and doing brightfield imaging. You will have to find yourself a DC lamp which won't flicker at high frequencies. If you are doing fluorescence work then I would suggest getting a new lamp since they tend to fluctuate in intensity as they get older. Other things to consider might be the trigger going to the camera or the speed with which the camera can be triggered may be less than your IPLab is sending. I don't really have enough information here to help you (eg: what kind of camera, type of controlling card etc...) My two cents worth. At 04:51 PM 6/6/2000 +0000, Maris Handley wrote: > >Hello Everyone, > >We are doing time lapse experiments with a Nikon microscope (using IPLab software). >When we play the movie back we are seeing large changes in light intensity (almost >random...not getting brighter continuously or dimmer continuously). Our exposure time >isn't being changed. I believe this is a problem with the light source stability, >but is there something else that might be causing this? Has anyone else seen this, >and what did you do to solve the problem? > >Thank you for any help, >Maris > > >Maris Handley >Dana-Farber Cancer Institute >Boston, MA 02115 >(617)632-3179 > > - Derek Schulze Flow Cytometry and Confocal Microscopy Core Facility Cancer Research Labs Queen's University Kingston, Ontario Canada http://meds.queensu.ca/medicine/crl/
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