An image analysis question

From: Maris Handley (maris_handley@dfci.harvard.edu)
Date: Tue Jun 06 2000 - 11:51:38 EST


Hello Everyone,

We are doing time lapse experiments with a Nikon microscope (using IPLab software).
When we play the movie back we are seeing large changes in light intensity (almost
random...not getting brighter continuously or dimmer continuously).  Our exposure time
isn't being changed.  I believe this is a problem with the light source stability,
but is there something else that might be causing this?  Has anyone else seen this,
and what did you do to solve the problem?

Thank you for any help,
Maris


Maris Handley
Dana-Farber Cancer Institute
Boston, MA 02115
(617)632-3179



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