Does this mean that RED670 has a "bright" emission intensity? Do cells
binding the SA-RED670 conjugate through the biotin moiety have a greater
separation from the basal level of nonspecifically stained cells due to this
increased brightness relative to other streptavidin-fluorochrome conjugates?
Kevin G. Waddick, Ph.D.
Parker Hughes Institute
> assay system using a fluorochrome named Sunnyvale Red. It excites at 488
> >nm and has a emission wavelength of 670 nm.
> >
> >Dixie Polakoff
> >FACS operator
> >Protein Design Labs
> >(510) 574-1528
> >
> >
> >
>
> Greetings Dixie,
>
> Sounds to me that this might be 'RED670'; I have used Streptavidin-RED670
> for the enumeration of G-CSF receptors via biotinylated G-CSF. I estimate
> that this fluorochrome is capable of reporting <100 receptors.
> It is available from GibcoBRL cat no.19543-016
>
> regards
>
> Arnold
>
> _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
> Arnold Richard Pizzey
> Department of Haematology
> Royal Free and University College London Medical School
> 98 Chenies Mews
> London WC1E 6HX
> U.K
>
> voice: +44 020-7679-6234
> Fax: +44 020-7679-6222
> email: a.pizzey@ucl.ac.uk
> _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
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