Greetings Kevin, I can only compare 'RED670' against the 'SpectralRed' conjugated 'Neutralite Avidin' (Southern Biotechnology Associates 7200-13), I have used both for G-CSF receptor enumeration and also for general antibody staining, my conclusion is that, the RED670 conjugate appears to be inherently brighter (both flurochromes titrated to supramaximal doses) in both applications with a fluorescence intensity up to a log higher than 'SpectralRed' in the same system. However, in some conditions- particulay when working with murine cells, the streptavidin-fluorochrome conjugates are less than ideal as they seem to bind directly to the cell membrane giving a high background. regards, Arnold > Greetings Dixie, > > Sounds to me that this might be 'RED670'; I have used Streptavidin-RED670 > for the enumeration of G-CSF receptors via biotinylated G-CSF. I estimate > that this fluorochrome is capable of reporting <100 receptors. > It is available from GibcoBRL cat no.19543-016 > > regards > > Arnold > Does this mean that RED670 has a "bright" emission intensity? Do cells >binding the SA-RED670 conjugate through the biotin moiety have a greater >separation from the basal level of nonspecifically stained cells due to this >increased brightness relative to other streptavidin-fluorochrome conjugates? > >Kevin G. Waddick, Ph.D. >Parker Hughes Institute _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ Arnold Richard Pizzey Department of Haematology Royal Free and University College London Medical School 98 Chenies Mews London WC1E 6HX U.K voice: +44 020-7679-6234 Fax: +44 020-7679-6222 email: a.pizzey@ucl.ac.uk _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
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