Dear Flowers, We are currently setting up a protocol to run malaria infected red cells on a coulter XL II. We have different markers we want to study using labeled antibodies. One of these cromogens/colours conjugated to these antibodies is Alexa Red. 1) Can Alexa Red be excited by a 488nm argon Laser? 2) Alexa red has a wide wavelength from 550 - >> 700. We are not sure whether to use the 575 , 620 or 675 filters in order to avoid overlap in 575 as we also use Ethidium Bromide to stain the parasite and use FL2 575 wavelength filter. 3) Is it possible for Alexa red to be excited by secondary emission . Any ideas? OSCAR K. KAI KEMRI(CGMRC)/WTRL IMMUNOLOGY DEPT P.O BOX 230 KILIFI KENYA Tel. 254 0125 22063 or 22535 or 25043 Fax. 254 0125 22390 email:Okai@kilifi.mimcom.net
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