Sounds familiar. We had the same problem with lock-up/crashes on four different computers which usually correlated a pending print job. We were using Quadra 650's on FACScans, a Vantage and a workstation (with no associated cytometer) while running CellQuest 3.1 with OS 7.6. A BD computer specialist came and spent several days trying to diagnose the problem with no success. Our final solution was to put the Vantage system back on CQ 1.2 which works fine. The workstation has since been replaced with a G3 which seems to have solved that problem; the two systems on the FACScans have improved (but not perfect) after removing all other software and closing any network connections while running CQ. This occured in the fall/winter of 1998 and there was discussion about relating to this on the bulletin board if you look in the archives. Wayne F. Green, Ph.D. HCI Flow Cytometry Core Lab wayne.green@hci.utah.edu Phone (801) 585-7382 -----Original Message----- From: Abby Kelliher [mailto:allena@helix.mgh.harvard.edu] Sent: Thursday, March 30, 2000 2:55 PM To: cyto-inbox Subject: Cellquest crashing Hello - I have recently begun having a very annoying problem with my Mac on my FacsCalibur. Several times a day, when I try to print I get an error message which reads something like: Not enough memory, close some views and try again. It is usually the only document open and it is a normal (usual file size which for years I have not had this problem with) file size. The computer then freezes up. The only way for me to get it back is for me to unplug the computer from the wall. I have been working with BD for a couple of days on this problem, but we have been unable to solve it yet. We have tried: 1)rebuilding the desktop with the extensions off 2)rebuilding the desktop with the extensions on 3) throwing out the finder preferences and rebuilding the desktop 4) run disk first aid from the Mac operating system cd (finds an error everytime I run it and supposedly repairs it 5) upped the memory allotment to cellquest 6) upped the disk cache It appears I was having a problem with the network as well, but I don't think the two problems are necessarily related. A few minutes ago, the worklist manager just quit on its own (while running) and left cellquest and the loader manager open but uncontrollable. When I tried a force quit, the computer locked up on me. I have had it freeze up on two other occasions while acquiring data. If anyone has any suggestions, I would GREATLY appreciate hearing them. I am at my wits end right now. Thanks in advance! Abby Abby Kelliher Clinical Flow Cytometry Laboratory Warren Bldg. Room 112 Massachusetts General Hospital 275 Charles St. Boston, MA 02114 617-726-8487
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