Unfortunately, our success in keeping the bead mix longer than a day hasn't been good. We use all four colors (FITC, PE, PERCP and APC) and the PERCP just doesn't have the stability on storage after mixing that the others have. I might add that this has been more of a recent observation in the last few months. Not long ago they seemed a lot more stable and we were able to use the mix for up to three days but never more. Again, this seems particularly true of the PERCP beads while the others are stable much longer. Haywood Pyle South Carolina Cancer Center Columbia, SC >From: "Jane S. Miller" <miller@medicine.tamu.edu> >To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> >Subject: Re: FACSComp QC >Date: Thu, 30 Mar 2000 13:23:34 -0600 > > >I run FACSComp each day that I run immunofluorescent samples. A mix of >beads will >keep a week or more. I don't make a new mix every time. Jane Miller > > >>> Tami Rosario <trosario@cellmate.cb.uga.edu> 03/29/00 12:55PM >>> > >I have a question for those of you who run FACSComp on the FACSCaliber >for QC. Do you run this each time you start up the cytometer or only >occasionally. I have been told that I should run FACSComp each day that >I run samples, but the beads are fairly expensive. Any alternatives? > >Thanks, >Tami > > ______________________________________________________ Get Your Private, Free Email at http://www.hotmail.com
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