Bill, P-Selectin expression on platelets is formaldehyde fixation stable. We routinely use Polysciences methanol free Ultrapure 10% formalin stock diluted in dPBS or 10mMHepes/saline. First dilute stock formalin to a 2% solution. You can add whole blood (we routinely use 3.2% citrate anticoagulant) 1:1 (vol:vol) to 2% formalin. Dilute fixed blood 1/10 prior to labeling. Good Luck, Lori Krueger ----- Original Message ----- From: William Ross <wgr8w@virginia.edu> To: cyto-inbox Sent: Friday, March 31, 2000 10:56 AM Subject: platelet fixation > > does anyone no of a good platelet fixation procedure for the study of > selectin expression? It is a study of myocardial infarction patients and > timing the heart attacks is difficult and we're trying to fix the purified > prep for later analysis. > > thanks in advance. > > Bill Ross > > William (Bill) Ross > Director - FACS Core Facility > MR-4, Box G005 > University of Virginia School of Medicine > Charlottesville, VA 22908 > office (804) 982-1586 > fax (804) 924-1221 > e-mail:wgr8w@virginia.edu > >
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