Hello Alice, you said: >We have found that using distilled/de-ionized water in the sheath is >noisier than >buffer on our cytometers. After puzzling over the question of >contaminants in the >water and after some discussion with a few gurus, we have concluded that >the noise >is not from contaminated water but may result from light bouncing off the >refractive >index change at the water (sheath): buffer (sample core) interface. > >Any thoughts on this? I concur. This effect is compounded when you're looking at small things with log laser scatter. td -- ============================================================================== Thomas M. Delohery |Internet: t-delohery@ski.mskcc.org Supervisor, Flow Cytometry Core Facility | Phone: (212) 639-8729 Memorial Sloan-Kettering Cancer Center | Fax: (917) 432-2333 1275 York Ave. Box 98 | New York, NY 10021 | ==============================================================================
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